Helicases unwind the two parental DNA’s strands and creates the replication fork; single strand binding proteins keep the single strand from joining.
• DNA replication: a biological method occurring in all living organisms that is the basis for biological inheritance
• Helicase: It is an enzyme that may unwinds the DNA helix ahead of the replication machinery.
• Origin of replication: a specific sequence in a genome at which replication is started.
Steps of DNA replication:
• During initiation, proteins fix to the source of replication while helicase unwinds the DNA helix and two replication forks are formed at the source of replication.
• During elongation, a primer sequence is added with complementary RNA nucleotides, which are then substituted by DNA nucleotides.
• During elongation the leading strand is made constantly, while the lagging strand is made in portions called Okazaki fragments.
• During termination, primers are detached and substituted with new DNA nucleotides and the backbone is stuck down by DNA ligase.
Purpose of DNA replication:
The purpose of DNA replication is to produce two duplicate copies of a DNA molecule. This is essential for cell division during development or repair of damaged tissues. DNA replication ensures that each new cell receives its own replica of the DNA.
Level: High school
• DNA replication
• Origin of replication
• Steps of DNA replication
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